The purpose of this experimental study was to compare scanning electron microscopically healing process of each anastomotic sites after mechanical or conventional microvascular anastomoses of the femoral vein in rabbit. Six rabbits weighed about
2.5kg
were used. Mechanical microvascular anastomosis was done on left femoral vein, the opposite side, with the conventional method. The experimental animals were sacrificed at 3, 5, 7, 10, 14 and 28 days after anastomosis. Results obtained were as
follows.
Conventional method was presented that at 3 days, endothelial regeneration was not recognized and intima to intima contact was not achieved due to vessel overlapping. At 5 days, insertion site of the thread was partially covered with amorphous
substance. At 7 days, endothelial regenration has begun but the thread was largely exposed. At 1 days, active regeneration of endothelium was observed over the anastomotic site and threads. At 14 days, active endothelial regeneration was observed
but
still the uncovered thread was seen. At 28 days, the thread was completely covered with normal endothelial cells.
Mechanical method was presented that at 3 days, endothelial regeneration was not recognized but intima to intima contact was uniformal achieved. At 5 days, it was similar to 3 days' appearance and regeneration was not observed on the anastomotic
site.
At 7 days, endothelial regeneration has begun but depression of anastomotic site was seen. At 10 days, half of the anastomotic site was covered with endothelial cell. At 14 days, the site of anastomosis was completely invisible and fully
endothelialized
and then at 28 days, it was similar to 14 days' healing pattern and directions of endothelial cell was arranged with the flow of blood.
By the above observation, because endothelial regeneration of the mechanical method was occured more evenly at the anastomotic site tan conventional, and exposure of suture material on the intimal vessel surface might carry the risk for
thrombosis,
it
has been suggested that mechanical method be superior to conventional method.
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